Gene description for fepA
Gene name ferric enterobactin outer membrane transporter
Gene symbol fepA
Other names/aliases ECK0576
cbr
cbt
fep
feuB
Species Escherichia coli str. K-12 substr. MG1655
 Experiment description of studies that identified fepA in Escherichia coli

1
Experiment ID373
Identified moleculeprotein
Extracellular vesicle typeMembrane vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 17787032   
OrganismEscherichia coli
Experiment descriptionGlobal proteomic profiling of native outer membrane vesicles derived from Escherichia coli.
AuthorsLee EY, Bang JY, Park GW, Choi DS, Kang JS, Kim HJ, Park KS, Lee JO, Kim YK, Kwon KH, Kim KP, Gho YS.
Journal name Proteomics
Publication year2007
SampleEscherichia coli
Sample nameNormal-Escherichia coli DH5α
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

2
Experiment ID2244
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 6
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

3
Experiment ID2245
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 7
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID2246
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 8
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID2247
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 9
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID2248
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113 - Fraction 10
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID2251
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

8
Experiment ID2252
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

9
Experiment ID2253
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

10
Experiment ID2255
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

11
Experiment ID2256
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 36648227   
OrganismEscherichia coli
Experiment descriptionEscherichia coli-Derived Outer Membrane Vesicles Relay Inflammatory Responses to Macrophage-Derived Exosomes
AuthorsImamiya R, Shinohara A, Yakura D, Yamaguchi T, Ueda K, Oguro A, Minamiyama Y, Ichikawa H, Horiguchi Y, Osada-Oka M.
Journal name mBio
Publication year2023
SampleEscherichia coli
Sample nameE. coli strain K-12 BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

12
Experiment ID2207
Identified moleculeProtein
Extracellular vesicle typeOuter membrane vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36013250   
OrganismEscherichia coli
Experiment descriptionProteomic Profiling of Outer Membrane Vesicles Released by Escherichia coli LPS Mutants Defective in Heptose Biosynthesis
AuthorsChiu YK, Yin T, Lee YT, Chen SJ, Wang YC, Ma KH.
Journal name J Pers Med
Publication year2022
SampleEscherichia coli
Sample nameE. coli strain BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

13
Experiment ID2208
Identified moleculeProtein
Extracellular vesicle typeOuter membrane vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36013250   
OrganismEscherichia coli
Experiment descriptionProteomic Profiling of Outer Membrane Vesicles Released by Escherichia coli LPS Mutants Defective in Heptose Biosynthesis
AuthorsChiu YK, Yin T, Lee YT, Chen SJ, Wang YC, Ma KH.
Journal name J Pers Med
Publication year2022
SampleEscherichia coli
Sample nameE. coli strain BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

14
Experiment ID2209
Identified moleculeProtein
Extracellular vesicle typeOuter membrane vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36013250   
OrganismEscherichia coli
Experiment descriptionProteomic Profiling of Outer Membrane Vesicles Released by Escherichia coli LPS Mutants Defective in Heptose Biosynthesis
AuthorsChiu YK, Yin T, Lee YT, Chen SJ, Wang YC, Ma KH.
Journal name J Pers Med
Publication year2022
SampleEscherichia coli
Sample nameE. coli strain BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

15
Experiment ID2210
Identified moleculeProtein
Extracellular vesicle typeOuter membrane vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 36013250   
OrganismEscherichia coli
Experiment descriptionProteomic Profiling of Outer Membrane Vesicles Released by Escherichia coli LPS Mutants Defective in Heptose Biosynthesis
AuthorsChiu YK, Yin T, Lee YT, Chen SJ, Wang YC, Ma KH.
Journal name J Pers Med
Publication year2022
SampleEscherichia coli
Sample nameE. coli strain BW25113
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the study
Methods used in the studyMass spectrometry
EV-TRACK -

16
Experiment ID2219
Identified moleculeProtein
Extracellular vesicle typeOuter membrane vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 32854612   
OrganismEscherichia coli
Experiment descriptionProbiotic Escherichia coli Nissle 1917-derived outer membrane vesicles enhance immunomodulation and antimicrobial activity in RAW264.7 macrophages
AuthorsHu R, Lin H, Li J, Zhao Y, Wang M, Sun X, Min Y, Gao Y, Yang M.
Journal name BMC Microbiol
Publication year2020
SampleEscherichia coli
Sample nameE. coli strain Nissle 1917
Isolation/purification methodsCentrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density1.127-1.175 g/mL, 1.271-1.295 g/mL
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

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